Figure 6.

Western blots of lysates from subcellular fractions; determination of fractions containing different cytoskeletal components and nuclear proteins. By the use of detergent and salt solubilization of proteins (Lenk et al., 1977), fractionated cell lysates were probed by Western blotting for actin, tubulin, keratins (monoclonal antibody to bovine hoof keratin), or pur α. Actin and tubulin were extracted in the Soluble fractions, keratin was extracted in the Soluble and Ins Pellet fractions, and pur α was extracted in the Soluble fraction.