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Fractionation of ODC protein and of cytoskeletal components. 35S-labeled NHEK fractions were immunoprecipitated with ODC antibody or probed for actin, tubulin, or keratin by Western blotting, using the fractionation scheme detailed in Figure 8. ODC protein was present in all fractions, as was actin. Tubulin was found only in the S0 fractions, with keratin only in the P1 fractions.
