Fig. 2.

Plasma levels of E₂ in lean and HFD mice. Data are presented as means ± SE; n = 8 for 4-mo-old mice and n = 5–6 for 12-mo-old mice. **P < 0.01 and ***P < 0.001 for 4-mo-old mice vs. 12-mo-old mice (lean and HFD, respectively). *P < 0.05 for lean mice vs. lean + E₂ and ***P < 0.001 for HFD vs. HFD + E₂ mice.