Fig. 3.

Resveratrol stimulated hepatic AMPK activity in ethanol-fed mice. A: Western blots were performed using anti-phosphorylated-AMPKα (anti-p-AMPKα), anti-AMPKα, anti-p-AMPKβ, anti-AMPKβ, and anti-phosphorylated acetyl CoA carboxylase (P-ACC) antibodies from liver extracts of mice fed a low-fat diet, 200 mg·kg⁻¹·day⁻¹ resveratrol, or 400 mg·kg⁻¹·day⁻¹ resveratrol with or without ethanol. β-Actin protein band was used to confirm equal loading and to normalize the data. B: relative levels of p-AMPKα, AMPKα, p-AMPKβ, AMPKβ, and P-ACC. Western blots were quantified by a PhosphorImager and MultiAnalyst (Bio-Rad) software analysis. All data are expressed as means ± SD; n = 4–8 animals. Means without a common letter differ, P < 0.05.