Fig. 10.

Prednisolone inhibits TNF-α-induced nuclear translocation of NF-κB in PASMC. Growth-arrested PASMC were incubated with or without TNF-α for 0.5 h and/or prednisolone for 24 h. A: Western blot analysis shows changes in the expression level of p65 and p50 in the cytoplasm (left) and in the nuclear fraction (right) of cells treated with TNF-α, TNF-α with prednisolone, or prednisolone alone. B and C: summarized data (n = 3 experiments) show the average protein level of p65 (left) and p50 (right), normalized to the protein level of GAPDH or histone. *P < 0.05 vs. control, **P < 0.01 vs. TNF-α.