Table 1.

Oligonucleotide sequences of the primers used for RT-PCR

Standard Names (Accession No.)	Size, bp	Predicted Sense/Antisense	Location (nt.)	Gene (Chrom.)
TRPC1 (X89066)		For regular PCR:		3q23
	369	5′-GATTTTGGAAAATTTCTTGGGATGT-3′/	1659–2027
		5′-TGATAGCAAATCATGAAGACAAA-3′
		For real-time PCR:
	108	5′-GCACCTGCTTTGCTTTGTTC-3′/	1819–1926
		5′-TGACAGCTCCCACAAAGGAC-3′
TRPC3 (Y13758)		For regular PCR:		4q27
	318	5′-TGACTTCCGTTGTGCTCAAATATG-3′/	1909–2226
		5′-CCTTCTGAAGCCTTCTCCTTCTGC-3′
		For real-time PCR:
	203	5′-CGCTGCCCAATATCACAGTT-3′/	1255–1457
		5′-GAAGATGGACAGCATCCCAA-3′
TRPC4 (U40983)		For regular PCR:		13q13.3
	415	5′-TCTGCAAATATCTCTGGGAAGAATGC-3′/	2370–2784
		5′-AAGCTTTGTTCGTGCAAATTTCCATTC-3′
		For real-time PCR:
	161	5′-CTCGGATCCCATCACTTCAG-3′/	790–950
		5′-GGCGAGAGTTCTGATTCTGCT-3′
TRPC6 (AJ006276)		For regular PCR:		11q22.1
	438	5′-CTTTGAGGAGGGCAGAACACTT-3′/	2684–3121
		5′-AGGAGTTCATAGCGGAGACTTG-3′
		For real-time PCR:
	117	5′-CAGACAATGGCGGTCAAGTT-3′/	1626–1742
		5′-TGGTCCACGCATTATCTTCC-3′
GAPDH (AF261085)		For regular PCR:		12p13.31
	243	5′-GACAACGAATTTGGCTACAGC-3′/	1091–1333
		5′-GATGGTACATGACAAGGTGC-3′
		For real-time PCR:
	108	5′-ATGGGGAAGGTGAAGGTCG-3′/	149–256
		5′-GGGGTCATTGATGGCAACAATA-3′

The accession numbers in GenBank for the sequence used to design the primers are shown in parentheses. Chrom., chromosome.