Fig. 2.

P63 protein is present in rat type II cells and human A549 cells. Western blot analysis was performed on whole cell lysates from the rat L2 and the human A549 alveolar epithelial cell lines and the freshly isolated type II cells (T II) after 24 h in culture. After gel electrophoresis and transfer to nitrocellulose, the blots were probed with P63 Ab used previously (left; Ref. 17), newly prepared P63 Ab (middle), and preimmune (Pre-Imm.) rabbit serum (right). Arrowheads indicate the P63 protein band.