Fig. 4.

P63 localization in type II cells at the electron micrographic level. Type II cells were incubated with surfactant protein-A (SP-A; 1 μg/ml) for 2 h, fixed, and incubated with P63 Ab followed by gold-conjugated goat anti-rabbit Ab. D is enlargement of area (“d”) shown in C. Gold particles (10-nm size in A, C, and D; 15-nm size in B) marking P63 proteins were found in structures consistent with endoplasmic reticulum (ER) (A, short arrows; B, arrowheads), plasma membrane (PM) [A, C, and D (A and D, arrowheads)], and on microvilli [C and D (D, long arrows)]. LB, lamellar body; N, nucleus.