Fig. 1.
Effects of flagellin vs. forskolin on transepithelial voltage (Vt) and resistance (Rt) of Calu-3 cell monolayers incubated with equal [Cl−] in both apical and basolateral solutions. Calu-3 cells grown on filters were mounted in Ussing chambers with HCO3− and Cl−-containing Ringer bathing both sides of the epithelium. The time course of responses to adding Salmonella typhimurium flagellin (1 μg/ml) or forskolin (50 μM) to the apical solution on Vt and Rt are shown for single experiments (A and B). Equivalent currents (Icalc) were calculated from Ohm's Law in the steady-state control (con) and after flagellin (Flag; C) or forskolin (Fsk; D) treatment for 5 similar experiments in each case. Average values for Icalc in each condition are shown by the bold numbers.