Table 1.
Transepithelial permeability of Calu-3 monolayers to FITC-dextran (10-kDa mol wt) and lucifer yellow (457-mol wt)
| Condition |
Permeability, ×10−8 cm/s |
|
|---|---|---|
| Pdextran | Plucifer yellow | |
| Cl−/Cl− solutions | ||
| Control | 4.9±1.5 (3) | 9.4±0.7 (3) |
| Flagellin | 5.3±1.3 (3) | 4.9±2.3 (3) |
| IL-1β | 6.0±2.8 (3) | 9.3±1.3 (3) |
| Cl−/Cl−-free | ||
| Control | NA | 7.8±4.2 (3) |
| Flagellin | NA | 13.9±5.8 (3) |
| IL-1β | NA | 17.5±6.0 (3) |
Calu-3 monolayers were incubated with either Cl−-containing Ringer on both sides (Cl−/Cl−) or with Cl−-containing Ringer on the basolateral side and Cl−-free Ringer on the apical side (Cl−/Cl−-free) and either dextran or lucifer yellow (100 μM) on the apical side. Cells were left untreated or treated for 2 h with flagellin (10−6 g/ml) or IL-1β (10 ng/ml). Permeabilities were calculated from transepithelial fluxes of the probes into the basolateral solution during the last 60 min of treatment. Data are averages ± SD (n = number of different experiments). There were no significant differences (P > 0.05) for Pdextran and Plucifer yellow during control vs. flagellin or IL-1β treatments in either Cl−/Cl− or Cl−/Cl−-free conditions.