Fig. 1.

Folate perturbation protocols. Study 1: folate deletion and repletion. Six-week-old A/J and C57BL/6J females were placed on each of the 9 treatment plans with 8 replicate mice per treatment per strain. Study 2: folate depletion and repletion with additional genetic and dietary perturbations in C57BL/6J. C57BL/6J wild type, C57BL/6J wild type on choline supplementation, and ApoE knockout on the C57BL/6J background were all placed on folate depletion and repletion experimental treatments. Wild-type C57BL/6J were also placed on the control diet for both the depletion and repletion period and designated as controls. Eight replicate mice were used for each of the 8 treatment plans.