Figure 5.
RME-2 Western blot. Total C. elegans proteins were size separated by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed with anti-RME-2(EXT) affinity-purified antibodies. Identical results were observed with anti-RME-2(INT) affinity-purified antibodies (our unpublished findings). Equal protein loading was confirmed by Coomassie blue staining of duplicate gels (our unpublished findings).