Table 1.
Timeline of experiment 2
| Time | Model |
|
|---|---|---|
| LPS-induced SIRS (develops faster) | CLP-induced sepsis (develops slower) | |
| Day 0 | 1st pump implanted (delivers nicotine or saline during days 0–13) | 1st pump implanted (delivers nicotine or saline during days 0–13) |
| Day 13 | Telemetry probe implanted | Telemetry probe implanted |
| 1st pump removed | 1st pump removed | |
| 2nd pump implanted (delivers nicotine or saline during days 13–18) | 2nd pump implanted (delivers nicotine or saline during days 13–18) | |
| Temperature and activity recording started | CLP or sham surgery performed | |
| Temperature and activity recording started | ||
| Mortality recording started* | ||
| Day 15 | LPS or saline injected | |
| Mortality recording started* | ||
| Day 18 | Experiment ended | Experiment ended |
SIRS, systemic inflammatory response syndrome; CLP, cecal ligation and puncture.
*
In either model, deaths occurred on the 2nd and 3rd days after pump replacement (days 15 and 16, respectively), when the effects of acute nicotine and nicotine withdrawal are known to be maximal.