Table 3.
Estimated values of the kinetic parameters descriptive of rotenone-dicumarol-insensitive CoQ1 reduction and CoQ1H2 oxidation on passage through normoxic lungs, a measure of the relative precision of estimates of these parameters, and correlation coefficients between these parameters
| Parameters | Estimated Values | CV, % | Correlation Matrix | |||
|---|---|---|---|---|---|---|
| Vmax3, μmol/min | 0.86 | 20.1 | 1 | 0.76 | −0.06 | 0.16 |
| Km3a, μM | 65.6 | 77.4 | 1 | −0.08 | 0.56 | |
| Vmax4, μmol/min | 4.6 | 3.2 | 1 | 0.60 | ||
| Km4a, μM | 75.9 | 14.5 | 1 | |||
Vmax3 and Km3a are the respective maximum rate and apparent Michaelis-Menten constant for coenzyme Q1 (CoQ1) reduction via rotenone-dicumarol-insensitive reductase(s); Vmax4 and Km4a are the respective maximum rate and apparent Michaelis-Menten constant for CoQ1 hydroquinone (CoQ1H2) oxidation via complex III. Parameter values were estimated from the mean values of the normoxic data in Figs. 3 and 4C. The values of Vmax3, Km3a, Vmax4, and Km3a for hyperoxic lungs are assumed to be the same as those estimated for normoxic lungs. CV, asymptotic coefficient of variation.