Table 4.

Estimated values of the kinetic parameters descriptive of rotenone and dicumarol-sensitive CoQ₁ reduction on passage through normoxic and hyperoxic lungs, a measure of the relative precision of the estimates of these parameters, and correlation coefficients between these parameters

Parameters	Estimated Values	CV, %	Correlation Matrix
Vmax1 (normoxic), μmol/min	2.17	8.6	1	0.61	0.83	−0.53	−0.50
Vmax1 (hyperoxic), μmol/min	1.15 *	12.0		1	0.61	0.52	−0.38
Km1a, μM	33.7	42.3			1	−0.40	−0.56
Vmax2, μmol/min	1.17	11.5				1	0.77
Km2a, μM	13.4	93.6					1

V_max1 and K_m1a are the respective maximum rate and apparent Michaelis-Menten constant for CoQ₁ reduction via complex I; V_max2 and K_m2a are the respective maximum rate and apparent Michaelis-Menten constants for NAD(P)H-quinone oxidoreductase 1 (NOQ1)-mediated CoQ₁ reduction. Parameter values were estimated from the mean values of the normoxic and hyperoxic data shown in Figure 4, A, B, and D, with V_max1 as the only parameter affected by exposure to hyperoxia.

^*Significantly (likelihood ratio test; P < 0.005) different from the corresponding normoxic value.