Fig. 2.

Morphology of LC neurons in culture. A: GFP-LC neurons were immunoreactive (IR) for TH (scale bar, 20 μm). B: the cell bodies, nuclei, and dendritic processes of GFP-LC neurons were clearly discernable (scale bars, 20 μm). Left two images are TH immunofluorescence, and right two images are GFP fluorescence. C: this neuron was identified by visualizing expression of GFP (inset; scale bar, 20 μm), and TH immunohistochemistry allowed visualization of the dendritic field (scale bar, 100 μm). Although multiple processes were TH-IR, it is possible that some of the dendrites emanated from nearby overlapping cells. D: most LC neurons were found to coexpress both GFP and TH. Bar graph shows the number of cells that expressed GFP (green), were TH immunofluorescent (red), and were both GFP+ and TH-IR (yellow).