Table 1.
TaqMan gene expression assays used for real-time RT-PCR
| Gene | Symbol | Assay ID# |
|---|---|---|
| Steroidogenic acute regulatory protein | Star | Rn00580695_m1 |
| Cytochrome P-450, subfamily 11A | Cyp11a | Rn00568733_m1 |
| Cytochrome P-450, subfamily 21A, polypeptide 1 | Cyp21a1 | Rn00588996_g1 |
| Melanocortin 2 receptor (ACTH receptor) | Mc2r | Rn02082290_s1 |
| Low-density lipoprotein receptor | Ldlr | Rn00598438_m1 |
| Hypoxia-inducible factor 1α | Hif1a | Rn00577560_m1 |
| Corticotropin-releasing hormone receptor 1 | Crhr1 | Rn00578611_m1 |
| Proopiomelanocortin | Pomc | Rn00595020_m1 |
| FBJ murine osteosarcoma viral oncogene homolog | Fos | Rn02396759_m1 |
| Corticotropin-releasing hormone | Crh | Rn01462137_m1 |
| Glucocorticoid receptor (GR) | Nr3c1 | Rn00561369_m1 |
| Mineralocorticoid receptor (MR) | Nr3c2 | Rn00565562_m1 |
| Hydroxysteroid 11β dehydrogenase 1 | Hsd11b1 | Rn00567167_m1 |
| Hydroxysteroid 11β dehydrogenase 2 | Hsd11b2 | Rn00492539_m1 |
| Neuropeptide Y | Npy | Rn00561681_m1 |
| Neuropeptide Y receptor 1 | Npy1r | Rn01402912_g1 |
| Actin, β | Actb | Rn00667869_m1 |
Each gene expression assay consisted of a mix of sequence-specific forward/reverse primers and a FAM-labeled probe. Note: Primer/probe sets that cross an exon-exon boundary are designated “m1” at the end of the Assay ID#. Primer/probe sets designated “g1” cross an exon-exon boundary but may still detect genomic DNA, while an “s1” designation describes an assay target found within a single exon (i.e., detects genomic DNA). RNA samples used for assays with “g1” or “s1” designations were treated with DNase prior to PCR.