Table 3.

Immunoblot analyses of adrenal subcapsule protein expression in PD2 and PD8 pups exposed to acute hypoxia (8% O₂)

Cellular Fraction From Adrenal Subcapsules	Protein	Area Density, AU Postnatal Day 2			Area Density, AU Postnatal Day 8
		Baseline	4 h	P	Baseline	4 h	P
Mitochondria	StAR	4.47±0.27	5.60±0.99	0.314	8.04±0.48	8.97±1.14	0.492
	CYP11A	4.28±0.20	3.65±0.43	0.232	12.90±2.14	10.87±2.13	0.527
	β-actin	2.40±0.25	1.49±0.37	0.089	4.07±0.70	3.09±0.56	0.315
Membranes	LDLR	0.99±0.11	0.68±0.22	0.307	3.51±1.12	3.19±0.44	0.797
	β-actin	4.18±1.11	4.80±0.16	0.544	2.12±0.39	0.51±0.15*	0.008

Adrenal subcapsules were pooled (1 litter/sample) and immediately frozen. Comparisons between age groups were not performed due to a significant effect of hypoxia on the loading control (β-actin); n = 4 per cellular fraction for each protein of interest (at each age).

^*Significant difference with P < 0.05. See Table 1 for descriptions of abbreviations. Note that samples titled “Membranes” consist of plasma membranes and membranes from the following organelles: endoplasmic reticulum, microsomes, peroxisomes, Golgi apparatus, endosomes, and lysosomes. AU, arbitrary units; LDLR, low-density lipoprotein receptor.