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. 2008 Aug 6;295(4):F877–F887. doi: 10.1152/ajprenal.00449.2007

Fig. 1.

Fig. 1.

Dibutyryl cGMP (db-cGMP) decreases surface apical Na/K/2Cl cotransporter (NKCC2) levels in thick ascending limb of the loop of Henle (THALs). A: dose-response curve showing maximum inhibition by db-cGMP at 500 μM (n = 5 for each concentration, *P < 0.05 vs. basal). B: representative Western blotting showing surface NKCC2 levels in THALs treated with vehicle (basal, lane 1) and db-cGMP 500 μM (lane 2) and intracellular NKCC2 in the same samples vehicle, (basal, lane 3) and db-cGMP 500 μM (lane 4). The intracellular protein GAPDH is absent from the surface fraction (lanes 1 and 2) but strongly expressed in the intracellular fraction (lanes 3 and 4).