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. 2008 Aug 6;295(4):F877–F887. doi: 10.1152/ajprenal.00449.2007

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Fig. 2. — 8-Br-cGMP decreases surface NKCC2 levels in THALs. A: 20-min incubation with 8-Br-cGMP (500 μM) decreased surface NKCC2 by 25% (n = 5, *P < 0.02 vs. basal). B: representative Western blot showing surface NKCC2 levels in THALs treated with vehicle (basal; lane 1) and 8-Br-GMP (500 μM; lane 2) and intracellular NKCC2 in the same samples [vehicle, basal (lane 3) and 8-Br-GMP (500 μM; lane 4)]. The intracellular protein GAPDH is absent from the surface fraction (lanes 1 and 2) but strongly expressed in the intracellular proteins (lanes 3 and 4).