Fig. 3.

Effect of WNK3 and chimeras on K⁺-Cl⁻ cotransporter KCC2. A: oocytes were injected with water or KCC2 cRNA alone or together with WNK3, chimera 344, or chimera 334 cRNAs, as indicated. B: oocytes were injected with water or KCC2 cRNA alone or together with WNK3, chimera 433, or chimera 443 cRNAs, as indicated. ⁸⁶Rb⁺ uptake was assessed in hypotonic medium 3 days later in the presence (open bars) or absence (filled bars) of extracellular Cl⁻. *Significantly different from the uptake observed in oocytes injected with KCC2 cRNA alone.