Fig. 11.

Epithelial Na⁺ channel (ENaC) β-subunit density distribution (A) and abundance (B) in renal cortex of rats on 3-wk 0.4% NaCl (n = 5) or 4% NaCl (n = 5). In A, immunoreactivity is expressed as % of the total signal in all 12 fractions. In B, arbitrary density values are normalized to the amount of protein loaded on the gradient and then normalized to fraction 6 of NS within each pair for statistical analyses. Typical immunoblots of ENaC-β from a constant sample volume from each gradient fraction are shown. Values are means ± SD. *P < 0.05 vs. NS, assessed by ANOVA followed by paired Student's t-test.