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NKA α1 (A)- and β1 (B)-subunit density distribution in renal cortex of rats on 3-wk 0.4% NaCl (n = 5) or 4% NaCl (n = 5). Typical immunoblots of a constant sample volume from each gradient fraction are shown for each protein. Immunoreactivity is expressed as % of the total signal in all 12 fractions. Values are means ± SD. *P < 0.05 vs. NS, assessed by ANOVA followed by paired Student's t-test.
