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Density distribution of renal cortical NHE3 (A), DPPIV (B), myosin VI (C), NaPi-2 (D), APN (E), NKCC2 (F), NCC (G), and NKA β1-subunit (H) in rats on 0.4% NaCl (n = 4–5) or overnight 4% NaCl (n = 4–5). Typical immunoblots of a constant sample volume from each gradient fraction are shown for each protein. Immunoreactivity is expressed as % of the total signal in all 12 fractions. Values are means ± SD. *P < 0.05 vs. NS, assessed by ANOVA followed by unpaired Student's t-test.
