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. 2008 Oct 12;8:100. doi: 10.1186/1471-213X-8-100

Figure 3.

Figure 3

Expression-patterns of representative signaling transcripts during WBR. Whole-mount RNA in situ hybridization of Bl-Rap1A (A-C'), Bl-Cnot (D-F'), Bl-Slit (G-I'), Bl-STAT (J-L'), Bl-Rab-33 (M-O'), Bl-Bax inhibitor (P-R') and Bl-Mnk (S-U') on colonial, naïve and regenerating blood vessels at three and eight days post separation. Naïve ampullae of B. leachi colonies show no detectable staining of transcripts within blood cells (A, D, G, J, M, P, S). During WBR, specific staining of the transcripts is present within regenerating fragments in a population of large (12–15 μm) round cells, and is absent from morula cells (I', L', U' blue arrowheads) and lymphocyte-like cells (C', F', L', black arrowhead). Bl-Rap1A, Bl-Cnot and Bl-Slit at three days of regeneration, stained circulatory cells (B, E, H respectively, arrows) juxtaposed to the vessel epithelium (can be observed in J, L', O', green arrowheads). At eight days of regeneration, staining was present in cell conglomerates within peripheral sites (C, F, I arrows) and absent from regenerating buds (C, F). Bl-STAT and Bl-Rab-33 at three days of regeneration, stained cells within multiple niches (K, N respectively, arrows). At eight days of regeneration, staining is still absent from developing buds and their niches (L, O) but present within niches in proximity to vessel epithelium (L', O' green arrowheads). Bl-Bax inhibitor and Bl-Mnk are expressed differentially within niches. At three days of regeneration, Bl-Bax inhibitor is systemically expressed within most niches (Q, arrows), but at eight days of regeneration, is completely absent from degenerating niches (R, red arrowhead). At three days, Bl-Mnk displays a comprehensive expression within selected niches (T, arrows), while absent from neighboring sites. Later, Bl-Mnk expression is confined to small cell aggregates within distant niches (U, arrows) and absent from developing buds (U). Red rectangles in 8 d column correspond to the respective enlargements. Am, ampullae; b, bud. Scale bar represents 100 μm.