95F myosin genomic structure. The genomic structure of 95F myosin shows the relative position of P element insertions and the mmw14 deletion. 95F myosin exons are shown in black. P element 1 is P[w+; Gal4], which is inserted 5′ of the first exon yielding the C865 line. Imprecise excision of this P element resulted in a 1-kb deletion, mmw14, which extends from the P[w+; Gal4] insertion site into the flanking DNA. Insertion of P element 2, P[lacZ, ry+], yields the jaguar mutation. Based on PCR analysis with primers to exon 2 of 95F myosin and the P end, the distance of P element 2 is ∼4.5 kb from the initiator methionine of 95F myosin (our unpublished results).