Figure 3.

RT-PCR to detect transcription of 95F MHC in jar^mmw14homozygous third instar larvae. (A) p1027 was used as the reverse transcription primer in lanes 2 and 3. Amplification primer pairs are listed on the right. No products were present in the jar^mmw14homozygous third instar larvae when primer pair p1 and p1027 was used (lane 3), whereas the control wild-type (Oregon-R) larvae had a 1-kb product (lane 2). This suggests that no transcripts containing the first exon are present. Truncation of this exon does not disrupt transcription of the 95F MHC gene, because a 0.5-kb band is observed when using the primer pair p517 and p1027 to amplify the reverse transcriptional products (lane 5). The 0.5-kb band (lanes 4 and 5) was not due to genomic DNA contamination, because two bands were detected when the genomic DNA was used as the template, and p517 and p1027 were used as amplification primers (our unpublished results). (B) A schematic diagram shows the location of the 95F MHC primers used in this experiment.