FIG. 6.

DPPX siRNA weakens both the rate of rise and fall of action potentials in hippocampal CA1 neurons. A: examples traces of AP firing in DPPX siRNA (top) and negative control siRNA (bottom)-expressing CA1 pyramidal neurons (black traces). Overlaid are the 1st temporal derivatives showing reduced peak rates of rise and fall for DPPX siRNA-expressing neurons (DPPX siRNA in red, control siRNA in green). Traces to the right show the 1st AP aligned with its derivative. The bottom set of traces show the overlaid 1st derivatives for DPPX siRNA (red trace) and negative control siRNA (green trace). B: phase-plane plot of the action potentials shown in the inset for DPPX siRNA (red traces) and control siRNA (black traces). C: pooled data showing a significant reduction in both rates of rise and fall for APs in DPPX siRNA-expressing neurons 2–3 days after infection compared with control siRNA (*, P < 0.05, unpaired t-test). No differences were observed 1 day after infection. Error bars represent SE.