TABLE 1.
Expression level, voltage dependence, and kinetic properties of K+ currents in outside-out patch recordings from CA1 neurons of organotypic slice cultures DIV 5–8
|
Current Density (pA/patch) |
Activation
|
Inactivation
|
τinact, ms
|
τrecov, ms
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| Transient | Sustained | Vh | k | Vh | k | −20 mV | +40 mV | −100 mV | |
| Control† | 194 ± 22 (35) | 44 ± 06 (35) | −9.2 ± 2.3 (34) | 18.3 ± 0.7 (34) | −70.0 ± 1.1 (26) | 9.6 ± 0.6 (26) | 20.1 ± 1.7 (24) | 33.9 ± 3.7 (23) | 19.0 ± 1.5 (26) |
| siDPPX (days 2–3) | 211 ± 21 (55) | 66 ± 08 (55) | 3.9 ± 2.0* (28) | 22.9 ± 0.9* (28) | −64.6 ± 1.1* (36) | 9.7 ± 0.4 (36) | 29.6 ± 3.7* (26) | 31.1 ± 2.9 (26) | 31.8 ± 6.6* (12)# |
†
Control group includes recordings from 15 uninfected neurons and 20 infected with negative control siRNA numbers are in parentheses. Vh, k, average fit half-activation and inactivation voltages and slope; τinact, weighted sum of double-exponential fit; τrecov, single-exponential recovery fit for currents measured with a two-pulse protocol (steps from −100 to +40 mV);
*
P < 0.05, unpaired t-test;
#
, data from siDPPX day 2 only.