Figure 1.
Evidence for a chimeric origin of TEP1. Synonymous site divergence (KS) between TEP5, TEP6, and TEP1 s and r, calculated for 30 consecutive windows of coding DNA sequence. (A) Divergence between TEP5 and TEP6. (B) Divergence between TEP1 (s in blue, r in red) and TEP6. (C) Divergence between TEP1 and TEP5. (D) Divergence between TEP1s and TEP1r. In (D), note that the region of high divergence between TEP1s and TEP1r covers sites 2100–3700 and the TED domain is shown as a black bar, and that in (B and C) TEP1 is most similar to TEP6 at sites 100–1500, but is most similar to TEP5 at sites 2000–3200 bp; specifically in the region 100–1500 the divergence between TEP6 and TEP1 is KS = 0.87 (95% bounds 0.70–1.10), but in the region 2000–3200 this divergence drops to KS = 0.37 (0.30–0.46) which differ significantly (p < 0.001 [37]). Note also that within the divergent region, TEP1r is consistently more similar to TEP6 than is TEP1s (red line vs. blue line); for example the divergence between TEP1r and TEP6 between sites 2250 and 3250 is KS = 0.15 (0.10–0.20) but between TEP1s and TEP6 is KS = 0.27 (0.20–0.35). Regions in which the MaxChi test suggests there is significant evidence (p < 0.05) for recombination breakpoints between TEP1 and TEP5 or TEP6 are shown as grey bars. The graph has been truncated when Ks>2