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. Author manuscript; available in PMC: 2008 Oct 30.
Published in final edited form as: J Nucl Med. 2008 Aug 14;49(9):1401–1404. doi: 10.2967/jnumed.108.053009

FIGURE 2.

FIGURE 2

Screening of small-molecule library for antiangiogenic compounds with transgenic zebrafish. (A) Bright-field and fluorescent images of 2-d–postfertilization zebrafish treated with vehicle control or PTK787 at 10 μmol/L (positive control). Angiogenic blood vessel growth was inhibited in head and trunk of PTK787-treated embryos. (B) For quantification of vessel growth in embryos, trunk was manually isolated from fluorescent image. Automated algorithm counted intersegmental vessels (IV) and various branching arteries. DLAV = dorsal longitudinal anastomotic vessel; VA = vertebral artery. (C) LOPAC1280 library was collaboratively screened by Zygogen (blue points) and Emory University (green points). Positive hits (red points) were defined as compounds that caused inhibition of angiogenic vessel count by more than 3 SDs from plate average. (Reprinted with permission of (13).)