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. Author manuscript; available in PMC: 2008 Nov 30.
Published in final edited form as: Cell. 2008 May 30;133(5):841–851. doi: 10.1016/j.cell.2008.04.011

Figure 4. Effects of Arp2/3 and CP on shell growth.

Figure 4

A–F. Reactions were Latrunculin B/phalloidin arrested 60 seconds after mixing, fractionated and analyzed as described. Conditions: 5.8 µm ActA-coated beads mixed with 3 µM actin (5% TMR-labeled), and Arp2/3 and CP as indicated.

A. Representative shells after arrest, fractionation, and washing.

B. Shell thickness decreases with [Arp2/3] and increases with [CP]. Error bars represent standard deviation (average n=17).

C. Soluble actin concentration at the time of arrest decreases with increasing [CP].

D. Total amount of actin in the shells does not vary with [Arp2/3] or [CP].

E. Average actin density in the shells increases with [Arp2/3] and decreases with [CP]. Density was calculated by dividing the actin mass in (D) by the volume of the shells as derived from (B).

F. Total number filaments in the shells is independent of [Arp2/3] but increases with [CP].