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. Author manuscript; available in PMC: 2008 Nov 30.
Published in final edited form as: Cell. 2008 May 30;133(5):841–851. doi: 10.1016/j.cell.2008.04.011

Figure 5. The kinetics of shell growth is independent of the kinetics of actin assembly.

Figure 5

A–F. Aliquots from each reaction were Latrunculin B/phalloidin arrested 15, 30, 45, and 60 seconds after mixing, fractionated, and analyzed as described. Conditions: 5.8 µm ActA-coated beads mixed with 3 µM actin (5% TMR-labeled), and Arp2/3 and CP as indicated.

A. Representative shells after arrest, fractionation, and washing.

B. Rate of shell growth decreases with [Arp2/3] and increases with [CP]. Error bars represent standard deviation (average n=27).

C. Rate of actin assembly is independent of [Arp2/3] and [CP].

D. Rate of filament nucleation is independent of [Arp2/3] but increases with [CP]. Nucleation rate per square micron of bead surface was derived from the slopes of the time courses.

E. Two alternate mechanisms for increasing the density of a dendritic actin network imply different actin assembly kinetics. See text for details.