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Effects of rolipram and 8-Br-cAMP on morphologic changes induced by AVP in L6-C5 cells. L6-C5 cells were grown in complete medium and after 24 h shifted to serum-free medium without (a, c, and e), or with (b, d, and f) 0.1 μM AVP. Ten micromolar rolipram (c and d) or 100 μM 8-Br-cAMP (e and f) was simultaneously added. After 6 d of culture, cells were stained by May Grunwald-Giemsa. Myogenic differentiation was evidenced by the formation of multinucleated myotubes.
