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. 1999 Dec;10(12):4355–4367. doi: 10.1091/mbc.10.12.4355

Figure 9.

Figure 9

Effect of PDE4D3 overexpression on L6-C5 cell differentiation. L6-C5 myoblasts were transiently transfected in suspension by using Fugene 6 with 1 μg of pCMV5 expression vector containing rat PDE4D3 cDNA (Sette et al., 1994c) or the empty vector and plated in 10% FCS-containing medium. After 24 h, the medium was replaced by serum-free medium with or without addition of 0.1 μM AVP. (A) Immunofluorescence analysis of the expression of myogenin in transfected L6-C5 cells. Mock-transfected cells (a and c) and pCMV5-PDE4D3-transfected cells (b and d) were left untreated (a and b) or were treated with 0.1 μM AVP for 48 h (c and d). Myogenin was detected by using the anti-myogenin antibody F5D. (B) Immunochemical detection of myosin expression in transfected L6-C5 cells. Mock-transfected cells (a and c) and pCMV5-PDE4D3-transfected cells (b and d) were left untreated (a and b) or were treated with 0.1 μM AVP for 6 d (c and d). Myosin was detected by using the anti-myosin antibody MF20.