Table 1.
Effect of PDE4D3 overexpression on L6 cell differentiation (myosin accumulation)
| Culture medium | Mock transfected (ng/μg DNA) | PDE4D3 transfected (ng/μg DNA) |
|---|---|---|
| 1% BSA | 45 ± 2.25 | 100 ± 1.1a |
| 1% BSA + 0.1 μM AVP | 320 ± 25.3 | 530 ± 9.1a |
L6 myoblasts were transiently transfected in suspension by using Fugene 6 with 1 μg of the pCMV5-PDE4D3 plasmid or the empty vector and plated in 10% FCS medium. After 24 h, the medium was replaced as indicated, with or without addition of 0.1 μM AVP. After 6 d of culture, the cells were solubilized, and their myosin content was measured by ELISA. Results are the means ± SD of six measurements performed in two independent experiments.
a
Significantly different from mock-transfected cells, p < 0.01.