Figure 3.

(A) Western blotting analysis of brain tissue from the proband. Brain samples are treated with Proteinase K (+) at a concentration of 50 μg/ml or without Proteinase K (-) before electrophoresis. (Top) Western blot of brain homogenates without Proteinase K digestion. (Bottom) Western blot graph of brain homogenates with Proteinase K digestion. Each brain region is indicated at the bottom of the image. Molecular weight standards are shown on the right. (B). Neuropathological assays of occipital lobe. Hematoxylin and eosin staining (left) and immunohistochemistry with monoclonal antibody 3F4 (right) (×400).