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Phase-contrast microscopy shows that exposure of cholesterol (30 mol %)/POPC (70 mol %) unilamellar vesicles (∼200 nm at time zero) to PP1 and PP2, both of which are high vapor pressure PFCs, for 72 h results in giant spherical vesicles of sizes ∼20–40 μm, probably because of vesicle fusion. Giant vesicles are far fewer and smaller when exposed to PP4 and PP9; both are low vapor pressure PFCs. Bar, 10 μm.
