Temporal evolution of Laurdan's generalized polarization (GP) of POPC/cholesterol vesicles in the presence of (A) PP1, (B) PP2, (C) PP4, and (D) PP9. Open circles indicate vesicles incubated with PFC; solid circles indicate vesicles in absence of PFCs. (Insets) Details of first 8 h of GP profile. Three different stages described in text are indicated as I, II, and III.