FIGURE 2.

Sample whole cell measurements of I/V curves for the larger P−2′Δ α1 GlyR channels in different dilutions of LiCl, NaCl, and CsCl solutions. The experimental procedures and solutions are identical to those described for Fig. 1. As in Fig. 1, the ΔV_rev values for all the diluted solutions are positive, indicating that this mutant GlyR channel is also anion-selective (but less so than the WT GlyR). Again, the ΔV_rev values are greatest for the LiCl solutions and least for the CsCl solutions, indicating that the relative sequence of counterion permeabilities is Li⁺ < Na⁺ < Cs⁺, as observed for WT GlyR channels, but note that all the ΔV_rev shifts are reduced compared to the values for the smaller diameter WT GlyR channel.