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. 2008 Aug 1;95(10):4772–4789. doi: 10.1529/biophysj.108.131318

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Activation kinetics. FRET optical distance versus time, 15 C. (a) Ca²⁺-induced activation: change after rapid mixing of preformed binary TnC-TnI complex with Ca²⁺ solution (top dotted trace) or with buffer (mock injection, bottom trace). Empirical double exponential fit (green): (48% amplitude), (52%). Global model-based fit (red) with derived parameters in Table 1. (b) TnI-induced activation: change after rapid mixing of TnI into Ca²⁺-presaturated TnC. Empirical single exponential fit (green): τ⁻¹ = 305 s⁻¹. Global model-based fit (red) with derived parameters in Table 1.

Inline graphic — Activation kinetics. FRET optical distance versus time, 15 C. (a) Ca²⁺-induced activation: change after rapid mixing of preformed binary TnC-TnI complex with Ca²⁺ solution (top dotted trace) or with buffer (mock injection, bottom trace). Empirical double exponential fit (green): (48% amplitude), (52%). Global model-based fit (red) with derived parameters in Table 1. (b) TnI-induced activation: change after rapid mixing of TnI into Ca²⁺-presaturated TnC. Empirical single exponential fit (green): τ⁻¹ = 305 s⁻¹. Global model-based fit (red) with derived parameters in Table 1.