FIGURE 6.

Deactivation kinetics after Ca²⁺ removal, 15°C. FRET optical distance change after rapid mixing of Ca²⁺-saturated TnC-TnI with buffer containing Ca²⁺ chelator, EGTA. Empirical single-exponential fit (green, obscured by red trace): τ⁻¹ = 125 s⁻¹. Global model-based fit (red) with derived parameters in Table 1. (Inset) (Eq. 22), the normalized adiabatic projection of the global χ² hypersurface on a model parameter (labeled) axis.