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. 2008 Jul 25;95(10):4726–4736. doi: 10.1529/biophysj.108.134379

FIGURE 4.

FIGURE 4

(A) Influence of lysozyme labeling on protein-mediated aggregation of liposomes. The indicated protein concentrations of lysozyme and A488-lysozyme (D/P = 0.44) (open and solid symbols, respectively) were added to POPC/POPS 4:1 lipid vesicles (total lipid 215 μM), and the extent of light scatter by the suspensions was measured by absorbance at 360 nm. The lines are mere guides to the eye. Error bars are ± SD for duplicate measurements (lipid vesicles only and lipid vesicles plus acceptor). (B) Efficiency of FRET (Eq. 11) between A488-lysozyme (D/P = 0.44) and Rh-PE (Rh-PE/total lipid = 1:400) in 4:1 POPC/POPS LUVs (total lipid 215 μM) as a function of the concentration of added protein. The line is a mere guide to the eye.