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Quantification of IgA delivery to apical endosomes in RhoAV14 cells. [125I]IgA was internalized basolaterally for 10 min at 37°C, whereas WGA-HRP was cointernalized from the apical surface of RhoAV14 cells grown in the presence of 20 ng/ml DC (+DC) or 5 pg/ml DC (+5 pg/ml DC). Details of the DAB reaction and quantitation are given in MATERIALS AND METHODS. Values (mean ± SD; n = 3) are from a representative experiment.
