Figure 4.
Postendocytic fate of basolaterally internalized EGF in RhoAWT, RhoAV14, or RhoAN19 cells. [125I]EGF was internalized from the basolateral surface of cells for 10 min at 37°C, and the cells were quickly washed and then chased for 120 min. The percentage of total degraded ligand released from RhoAWT (A), RhoAV14 (B), or RhoAN19 (C) cells is shown. Values for transcytosis were as follows: RhoAWT + DC, 10.1 ± 0.4%; RhoAWT − DC, 11.1 ± 1.0%; RhoAV14 + DC, 9.0 ± 1.0%; RhoAV14 + 5 pg/ml DC, 11.5 ± 2.3%; RhoAN19 + DC, 9.2 ± 0.7%; RhoAN19 − DC, 12.5 ± 3.3%. Values for ligand recycling were as follows: RhoAWT + DC, 19.4 ± 1.2%; RhoAWT − DC, 17.9 ± 1.3%; RhoAV14 + DC, 22.7 ± 0.5%; RhoAV14 + 5 pg/ml DC, 24.6 ± 2.8%; RhoAN19 + DC, 16.8 ± 0.7%; RhoAN19 − DC, 17.1 ± 0.9%. Values for ligand remaining cell associated were as follows: RhoAWT + DC, 5.0 ± 0.8%; RhoAWT − DC, 4.8 ± 0.3%; RhoAV14 + DC, 2.8 ± 0.5%; RhoAV14 + 5 pg/ml DC, 6.6 ± 1.2%; RhoAN19 + DC, 13.2 ± 5.1%; RhoAN19 − DC, 10.3 ± 3.0%. Values (mean ± SD; n = 3) are from a representative experiment. (A and C) ■, +DC; ●, −DC. (B) ■, +DC; ●, 5 pg/ml DC.