Figure 6.
Postendocytic fate of basolaterally internalized IgA in RhoAWT, RhoAV14, or RhoAN19 cells. [125I]IgA was internalized from the basolateral surface of cells for 5 min at 37°C, and the cells were quickly washed and then chased for 120 min. The percentage of total ligand released basolaterally (recycled) or apically (transcytosed) in RhoAWT (A), RhoAV14 (B), or RhoAN19 (C) cells is shown. Values for degradation were as follows: RhoAWT + DC, 5.3 ± 0.7%; RhoAWT − DC, 5.8 ± 0.5%; RhoAV14 + DC, 5.8 ± 0.5%; RhoAV14 + 5 pg/ml DC, 13.6 ± 0.8%; RhoAN19 + DC, 6.3 ± 0.6%; RhoAN19 − DC, 6.4 ± 0.5%. Values for ligand remaining cell associated were as follows: RhoAWT + DC, 8.1 ± 0.2%; RhoAWT − DC, 9.1 ± 1.3%; RhoAV14 + DC, 5.7 ± 1.0%; RhoAV14 + 5 pg/ml DC, 19.4 ± 1.0%; RhoAN19 + DC, 2.5 ± 0.2%; RhoAN19 − DC, 3.0 ± 0.4%. Values (mean ± SD; n = 3) are from a representative experiment. (A and C) ■, +DC, transcytosed; ●, +DC, recycled; ▴, −DC, transcytosed; ♦, −DC, recycled. (B) ■, +DC, transcytosed; ●, +DC, recycled; ▴, 5 pg/ml DC, transcytosed; ♦, 5 pg/ml DC, recycled.