Table 1.
Kinetic parameters of liver and recombinant rat GNMT.
Analysis methods | Parameters | Proteins | |
---|---|---|---|
Liver GNMT | Recombinant WT GNMT | ||
Hill | Vmax, nmol/min·mg | 326 | 400 |
K0.5 (AdoMet), µM | 280 | 460 | |
Hill coefficient | 1.10 | 1.02 | |
kcat, min−1 | 42.3 | 51.9 | |
Michaelis-Menten | Km (Gly), mM | 1.9 | 1.2 |