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. 2008 Nov 14;4(11):e1000258. doi: 10.1371/journal.pgen.1000258

Figure 4. Both the p21 antisense transcript and Ago-1 are required for the regulation of p21 promoter-associated RNAs and the suppression of promoter activity.

Figure 4

(A) The antisense transcript, Bx332409, was silenced post-transcriptionally (shown in inset) with p21-322 or si-Bx332409 siRNAs (50 nM) in the presence or absence of Ago-1. In the absence of Ago-1, an enrichment of dTT reverse transcribed p21 promoter associated RNAs (pRNA) are observed. Averages from triplicate transfected cultures, standard deviations, and p values from paired T tests are shown. (B and C) The p21 sense transcript, coding mRNA, was silenced post-transcriptionally (shown in inset) with p21-52 or p21-858 siRNAs (50 nM) in the presence (B) or absence (C) of Ago-1. In the presence of Ago-1 and p21 antisense transcription (B) an increase in p21 antisense pRNAs are observed with a concomitant loss of p21 sense pRNAs. In the absence of Ago-1 however (C) a loss of antisense pRNAs and an enrichment for sense transcribed p21 pRNAs is observed. Averages, standard deviations, and p values from paired T tests are shown from triplicate transfected cultures.