|
For sequencing
|
| Intron2/3F2 |
TTCTGTGTTATATACAACGTG
|
|
| Exon3F |
TCCAGAACATTTGAAAACGAG
|
|
| Intron3/4R |
CACATGCATAGCTAATGTGGTAATG
|
|
| Intron3/4F |
AAATCGTGTGTTTATGGAATTGACG
|
|
| Exon4F |
TATCTCTTGAACCATATACACGCGC
|
|
| Exon4R |
GCGCGTGTATATGGTTCAAGAGATA
|
|
| Exon5F |
TATTTCTTCTAGAATGTCTGAG
|
|
| Exon5R |
TAGATAATGTACTGCCCACTTTGTG
|
|
| Intron5/6F |
ATATGTGCGTCTGCTTTTAACTACG
|
|
| Intron6/7F |
GCTGTCTCTCGCTAACAATACGACG
|
|
| Intron6/7R |
ACATTTTCGTCGTATTGTTAGCGAG
|
|
| Intron7/8F1 |
CAGCTTCACATAAATGGAAACAC
|
|
| Intron7/8F2 |
AGTGGTTTACGAAAGTGAGGC
|
tA:48 - elong: 4min - 40 cycles |
| Intron7/8R |
TAGTAACATTGGTCGTTCGTG
|
|
| Intron8/9F |
AATGAAATAGTGAAAGAATGTTCG
|
|
| Intron8/9R |
CTTTCACTATTTCATTCAACAACG
|
|
| Intron9/10F |
CATCGCGTTATTCACTTAGCC
|
|
| Intron9/10R |
TAAAGGTGGAATATGCCAAACTCAC
|
|
| Exon10F |
TGAAGCTCAACTCAGTAAGCTGAAC
|
|
| Exon10R |
AACTCATTATTTTGAATGTTCAGC
|
|
| Exon11R |
CTTGTATCGCCTTCGATTCCAATTC
|
|
| Exon11/12F |
GACAGATTCGCTTAGTGATGAAG
|
|
| Intron11/12R1 |
CTTCATCACTAAGCGAATCTGTC
|
|
| Intron11/12R2 |
GTTGCCTGAATTCACCATCTC
|
|
| Exon14F |
TTCTTAGCACTACCCAAAGATGAAC
|
|
| Exon14R |
TATTTGTTCATCTTTGGGTAGTGC
|
|
| Intron14/15R |
GTATAATTCCTAAATATCGC
|
|
| Exon15R |
TGACACAGAAAACTGTTAACGATCC
|
|
|
For PCR amplification
|
| Exon1F12 |
GGAAGAATGAACAAGAAAATTATTCTC
|
tA: 65°C - elong: 3 min - 40 cycles |
| Exon15R |
TGACACAGAAAACTGTTAACGATCC
|
|
| NestedExon1F |
TATNTTGCGCTGATGATAAG
|
tA: 46°C - elong: 3 min - 40 cycles |
| NestedExon15R |
ATCATAAACAAACACTGAGG
|
|
| Intron2/3F1 |
CACTTGTTCATAAACACGTGTCTTC
|
tA: 59,5°C - elong: 10 min - 40 cycles |
| Exon15R |
TGACACAGAAAACTGTTAACGATCC
|
|
| Intron2/3F1 |
CACTTGTTCATAAACACGTGTCTTC
|
tA: 60°C - elong: 45 s - 30 cycles |
| Exon4R |
GCGCGTGTATATGGTTCAAGAGATA
|
|
| Intron3/4F |
AAATCGTGTGTTTATGGAATTGACG
|
tA: 60°C - elong: 50 s - 30 cycles |
| Intron3/4(gr.2)R |
ATTCAAATCAGTGATTGGTGTTCAC
|
|
| Intron3/4(gr.3.4–5)R |
CATGAAAATGGGTTATTTGCTAGTG
|
|
| Intron3/4(gr.3.4–5)F |
CACTAGCAAATAACCCATTTTCATG
|
tA: 60°C - elong: 4 min - 30 cycles |
| Intron3/4F |
AAATCGTGTGTTTATGGAATTGACG
|
|
| Intron9/10R |
TAAAGGTGGAATATGCCAAACTCAC
|
|
| Intron5/6F |
ATATGTGCGTCTGCTTTTAACTACG
|
tA: 60°C - elong: 5 min - 30 cycles |
| Exon11R |
CTTGTATCGCCTTCGATTCCAATTC
|
|
| Intron5/6F |
ATATGTGCGTCTGCTTTTAACTACG
|
tA: 60°C - elong: 50 s - 30 cycles |
| Intron6/7R |
ACATTTTCGTCGTATTGTTAGCGAG
|
|
| Exon10F |
TGAAGCTCAACTCAGTAAGCTGAAC
|
tA: 60°C - elong: 25 s - 30 cycles |
| Exon11R |
CTTGTATCGCCTTCGATTCCAATTC
|
|
| Exon3F |
TCCAGAACATTTGAAAACGAG
|
tA: 58°C - elong: 20 s - 30 cycles |
| Intron3/4R |
CACATGCATAGCTAATGTGGTAATG
|
|
|
For intermingled repeats amplification
|
| r1.F2 |
GCTCTCACATTTCAGATGACTAT
|
tA: 60°C - elong: 1 min - 30 cycles |
| r1.R2 |
AACTCACCTGTTGGTTCGCTC
|
|
| r2.F2 |
TCTCACATTTCAGGTGACCTC
|
|
| r2.R2 |
AACTCACCTGTGGGTTTGTCTG
|
|
|
For real time quantitative PCR
|
| Exon7F2 |
TATACGGAACAGACATGAGC
|
|
| Exon7R |
ACATTGGTCGTTCGTG
|
|
| Src.F1 |
TACGCTACCAACCCTGT
|
|
| Src.R1 |
CAAACTGCCCTTCTGT
|
|
