Table 1.
Strains and plasmids used in this study
| Bacterial strains | Genotype and relevant characteristics | Source or reference |
|---|---|---|
| Vibrio anguillarum 775 (pJM1) | Wild type | (Crosa, et al., 1980) |
| Vibrio anguillarum CC9-16(pJHC9-16) | Anguibactin-deficient, iron transport-proficient | (Walter, et al., 1983) |
| Vibrio anguillarum CC9-8(pJHC9-8) | Anguibactin-deficient, iron transport-deficient | (Walter, et al., 1983) |
| Vibrio anguillarum 775(pJM1-120) | 775 carrying pJM1 with Tn3::Ho-Ho1 insertion in angN | This study |
| Vibrio anguillarum 775(pJM1-4) | 775 carrying pJM1 with Tn3::Ho-Ho1 insertion in angR | Laboratory strain |
| Vibrio anguillarum H775-3 | Plasmidless derivative of 775 | (Crosa, et al., 1980) |
| Vibrio anguillarum H775-3(pJHC-T2612) | Plasmidless derivative of 775 carrying pJHC-T2612 (TAF−) | (Tolmasky, et al., 1988) |
| Escherichia coli XL1 blue | recA1, endA1, gyrA46, thi, hsdR17, supE44, relA, lacF′ [proAB+, lacIq, lacZM15 Tn10 (Tetr)] | Stratagene |
| Escherichia coli HB101 | supE44 hsd20 (r−B m−B) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | (Boyer and Roulland- Dussoix, 1969) |
|
| ||
| Plasmids | Relevant characteristics | Source or reference |
|
| ||
| pJM1 | Indigenous plasmid in strain 775 | (Crosa, et al., 1980) |
| pJHC-T2612 | Recombinant clone carrying a 24 kb region of pJM1cloned in pVK102, Tcr | (Tolmasky, et al., 1988) |
| pJHC-T2612#120 | pJHC-T2612 with a Tn3::Ho-Ho1 insertion in angN, Tcr, Apr | (Tolmasky, et al., 1988) |
| pJHC9-8 | pJM1 derivative carrying only the TAF region | (Tolmasky, et al., 1988) |
| pJHC9-16 | pJM1 derivative carrying TAF and transport genes, | (Tolmasky, et al., 1988) |
| pPH1JI | Plasmid with RP4 ori, incompatible with pJHC-T2612, Gmr | (Hirsch and Beringer, 1984) |
| pRK2073 | Helper plasmid for conjugation, Tpr, Tra+ | (Figurski and Helinski, 1979) |
| pBluescript SK+ | Cloning vector, Apr | Stratagene |
| pCR®-BluntII-TOPO® | Cloning vector, Kmr | Invitrogen |
| pBR325 | Cloning vector, Tcr, Cmr, Apr | (Bolivar, 1978) |
| pBR325-M200 | Cloning vector derived from pBR325, Tcr, Cmr, Aps | (Di Lorenzo, et al., 2004) |
| pTL61T | lacZ reporter vector, Apr | (Linn and St Pierre, 1990) |
| pMDL6 | 3.2 kb PCR fragment from pJM1 containing the angN gene cloned in pCR®- BluntII-TOPO® | This study |
| pMDL6-D133A | pMDL6 with mutation D133A in Cy1 domain | This study |
| pMDL6-D138A | pMDL6 with mutation D138A in Cy1 domain | This study |
| pMDL6-D575A | pMDL6 with mutation D575A in Cy2 domain | This study |
| pMDL6-D580A | pMDL6 with mutation D580A in Cy2 domain | This study |
| pMDL6-D133A/D575A | pMDL6 with mutation D133A in Cy1 domain and D575A in Cy2 domain | This study |
| pMDL6-D138A/D580A | pMDL6 with mutation D138A in Cy1 domain and D580A in Cy2 domain | This study |
| pMDL30 | ClaI-BamHI fragment from pMDL6 cloned in pBR325-M200 | This study |
| pCy1D1-D133A | ClaI-BamHI fragment from pMDL6-D133A cloned in pBR325-M200 | This study |
| pCy1D2-D138A | ClaI-BamHI fragment from pMDL6-D138A cloned in pBR325-M200 | This study |
| pCy2D1-D575A | ClaI-BamHI fragment from pMDL6-D575A cloned in pBR325-M200 | This study |
| pCy2D2-D580A | ClaI-BamHI fragment from pMDL6-D580A cloned in pBR325-M200 | This study |
| pCy1/2D1-D133A/D575A | ClaI-BamHI fragment from pMDL6-D133A/D575A cloned in pBR325- M200 | This study |
| pCy1/2D2-D138A/D580A | ClaI-BamHI fragment from pMDL6-D138A/D580A cloned in pBR325- M200 | This study |
| pCRII-PangN | 350 bp PCR fragment from pJM1 containing the upstream region of the angN gene cloned in pCR®-BluntII-TOPO® | This study |
| pHNN-2 | XhoI-PstI fragment from pCRII-PangN cloned in pTL61T | This study |
| pSC50 | 125 bp Sau3AI fragment of the fatB gene cloned in pBluescript SK+ | This study |
| pQSH6 | 415 bp SalI-ClaI fragment of the aroC gene cloned in pBluescript SK+ | (Di Lorenzo, et al., 2004) |